ABSTRACT
The separation efficiency of modern liquid chromatography (LP) has been developed to a very high level by the introduction of very selective as well as common-purpose stationary phases, with very small particle sizes resulting in columns with large plate numbers per meter. The development of LC equipment followed up the achievements in column technology, but the weakest part of high-performance liquid chromatography equipment is still the detection system. If the derivation is performed on-line after the chromatographic column, one speaks of post-column derivation or generally of chemical reaction detection (CRD). The chromatographic system must be optimized according to general practice, and the total peak broadening of this system should be determined and optimized before connecting the column with the CRD. The reagent should be delivered continuously, pulselessly, and at a constant flow rate. Usually, typical chromatographic pistons or membrane pumps can be used.
