ABSTRACT
This chapter describes succinctly for the practicing chemical, biochemical, and process engineers and research and development scientists and investigators the powerfulness of biospecific affinity chromatography to obtain highly purified proteins and enzymes that can be used for either in vivo or in vitro applications. The selectivity of biospecific affinity chromatography has proven to be important in the isolation and purification to homogeneity of newly discovered proteins and enzymes. Many types of matrices have been evaluated for use in affinity chromatography ranging from silica glass to polyacrylamide to agarose. The theoretical quantitation of affinity chromatography has been reported recently that can be used in the future to predict spacer-arm length, and ligand length and type for the protein to be used with certain physicochemical properties. The application of salt-promoted adsorption chromatography has been limited.
