ABSTRACT
Planar chromatography is still an important system of analysis and is widely used for the control of pharmaceutical products, in the sense of analysis of the production cycle as well as for the detection of complex matrixes, especially in toxicological investigations. Planar chromatography continues to be used in the qualitative analysis of alkaloids in the control phase of both pharmaceutical formulations and vegetable drugs. The detection limit for the glycyrrhizin is 5.06 ng for deposition and it is consequently applicable to quality control of pharmaceuticals and food products containing this substance. Thin-layer chromatography (TLC) has, for a long time, been a widely accepted methodology for the separation of multiple classes of antibiotics. The work underlines the function of the TLC as a preparative tool in the quantification of digoxin and derivatives. The buffered mixture is deposited on silica gel TLC plates and the chromatography is carried out with benzene–methanol.
