ABSTRACT
This introduction presents an overview of the key concepts discussed in the subsequent chapters of this book. The book discusses the concept of plant tissue culture. It examines various methods to prepare medium, and provides complete and logical examples of how to make solutions and dilutions and to accomplish sterile culture work. The book then introduces the three types of commonly used culture regeneration systems. It also focuses on the developmental processes and significance of "nonzygotic embryogenesis". Nonzygotic embryogenesis covers all instances where embryogenesis occurs outside of the normal developmental pathway found in the seed. The book demonstrates the use of plant protoplasts. Protoplasts are plant cells from which the cell wall has been enzymatically removed, making the cells amenable to cell fusion and other methods of germplasm manipulation. The book then explores the genetic transformation, which is a current hot topic in agriculture. It also describes the use of cryopreservation of germplasm.
