ABSTRACT
The use of Evaporative Light Scattering Detection (ELSD) which is recognized as universal for liquid chromatography is growing in packed column supercritical fluid chromatography (SFC). Moreover, the physical state of scattering particles can influence the response of the detector. Initially, the interfacing was composed of solely of a capillary restrictor to produce the back-pressure. The coupling permits gradient elution to be obtained under constant pressure. ELS detection is a highly suitable method for the analysis of various compounds with or without chromophore and as its response factor is similar for many compounds, it is currently being investigated for the analysis of a wide range of organic compounds. The field of applications of this coupling is wide: from a polar solutes such as alkanes with neat carbon dioxide up to high polarity solutes such as carbohydrates and amino-acids requiring polar modifier.
