ABSTRACT
The ability to detect virus infection in plants is important for predicting and monitoring plant virus epidemics. The presence of the virus capsid will not inhibit the binding of viral nucleic acid to the membrane." However, for successful binding, the nucleic acid must not have an extensive secondary structure. Identification of viruses is required in many aspects of plant pathology, for example, in the prediction of plant diseases in crops, plant quarantine control, prevention of infection in planting stock, and disease monitoring. One of the most important contributions of the tissue-print hybridization technique is its ability to show the localization of the virus in the plant. The tissue-print hybridization technique can essentially be divided into two parts: transfer of the viral nucleic acid from the plant tissue directly onto a membrane and hybridization of the printed membrane with a nucleic acid probe reporter system.
