ABSTRACT
The efficiency of high-performance immunoaffinity chromatography (HPIAC) separations depends on the selectivity of immobilized antibodies to perform the separation. The antibody is bound to an inert matrix, which forms the packing material of the column. The HPIAC separation is performed in two phases: a primary isolation phase, followed by a secondary elution phase. In the primary phase, a solution containing the material to be isolated is passed over the immobilized antibody, which reacts with the antigen forming an immobilized antibody-antigen complex. The chapter shows that the two most common techniques for recovering isolated materials from HPIAC columns are acid or chaotropic ion elution, although other techniques. The second phase of HPIAC is the elution or recovery phase which entails breaking the intermolecular forces which are involved in the antibody/antigen complex. A modification of the glass bead is binding a coating of bacterial coat proteins to the surface of the beads.
