ABSTRACT
The decapeptide luteinizing hormone-releasing hormone (LHRH) and its analogs are a major area of research. The amino acid content of a peptide is a major factor in determining whether ultraviolet (UV), electrochemical (EC), or fluorescence detection will be the most sensitive. If two peptides coelute during HPLC and one is electroactive while the other is not, the compounds can be "resolved" by determining one by EC and both by UV or fluorescence. If more sensitivity or selectivity is needed than can be obtained from the unaltered peptide, derivatization followed by fluorescence or EC detection is an option. For the purposes of UV and fluorescence detection, the organic solvent should have a low fluorescence and UV cut-off. To compare detector sensitivity, the "figure of merit" (FOM) suggested by D. K. Roe will be used. Using the FOM, the most sensitive detector can be selected for gradient analysis.
