ABSTRACT
The term chromatography is usually associated with separations carried out in the elution mode when a relatively small amount of a mixture is applied to the column at the inlet and the components are separated into individual bands as they move down the column with different velocities upon the action of the eluent flow. In classical displacement chromatography, the feed is introduced into the column that is equilibrated with a mobile phase called the carrier, all the components of which bind more weakly to the stationary phase than any of the feed components. Indeed, as the considerable body of work attests, displacement has proved itself as a powerful means for the purification of peptides and is likely to continue to be of increasing importance in this area. As research continues and appropriate displacers are identified or synthesized, displacement is likely to become an important technique in preparative protein purifications as well.
