ABSTRACT
Protein separation is a central technology in modern biological science. Although the efficiency of protein separation has been greatly improved by recent progress in electrophoresis and high-performance liquid chromatography (HPLC) techniques, more systematic and universal methods for total separation and analysis of proteins are required not only to reduce the time and labor for purifying proteins, but also to elucidate the molecular basis of various biological events or to determine the basis for the morphological and functional specificities of cells or tissues. Likewise, systematic peptide separation is important in order to survey and to isolate physiologically active peptides, and to separate peptide fragments of a protein in the strategy of protein sequence analysis. The automated 2D-HPLC technique is very powerful for systematic protein and peptide separations, as could be demonstrated in the protein mapping of bovine cerebellum and in the peptide mapping of ceruloplasmin.
