ABSTRACT
The System 6300 series of amino acid analyzers are dedicated instruments using cation-exchange with postcolumn ninhydrin derivatization for separation and quantification of amino-containing components. This instrument represents a refinement of the D. H. Spackman, W. H. Stein, and S. Moore technology that offers many advantages over alternative separation/quantification technologies. The System 6300 simultaneously monitors primary amines at 570 nm, secondary amines at 440 nm, and a reference channel at 690 nm. Many patented design features in the colorimeter and other components of the instrument ensure maximum sensitivity and resolution. Other features that contribute to the performance of the System 6300 are the narrow-bore column, selection of up to six buffers, column temperature control, and refrigerated storage of buffers, reagent, and samples. The major advantage of the System 6300 is the "turnkey" concept of instrument use. Documented applications methods allow separation of all the amino acids required by the protein chemist with little operator intervention.
