ABSTRACT
This chapter addresses the separation technique known as liquid chromatography or, more specifically, high-performance liquid chromatography and shows the general outline of the various chromatographic techniques. It discusses the types of chromatography: ion-exchange chromatography, partition chromatography, reversed-phase chromatography, hydrophobic interaction chromatography, hydrophilic interaction chromatography, and affinity chromatography. The chapter explains the list of chromatographic terms, determination of peak asymmetry, and various chromatographic peak parameters. The amount of stationary phase coated or bonded onto a solid support. In liquid- liquid chromatography, the milligram amount of liquid phase per gram of packing. There are many modes of chromatography, for example, size- exclusion, reversed-phase, hydrophobic interaction, hydrophilic interaction, and ion-exchange. The process of using liquid chromatography to isolate a sufficient amount of material for other experimental or functional purposes. In ion-exchange chromatography, regeneration involves replacing ions taken up in the exchange process with the original ions that occupied the ion-exchange sites.
