ABSTRACT
The amniotic layer was carefully separated from human placenta and purified. 8 × 10 cm pieces of the amniotic layer were soaked in a solution of an antibacterial agent and subsequently cross-linked with glutaraldehyde. The amount of drug bound was estimated and found to be 40 microgrammes of sulfadiazine per 10 cm-2 and 120 microgrammes silver per 10 cm2 indicating that silver was bound to the protein as well. The efficacy of the modified amniotic layer was tested by inflicting artificial cut wounds in the rabbits. The modified amniotic layers were inserted in the incision wounds, and the biopsies were taken at regular intervals for histopathological studies. The amniotic layers enhanced the healing; it appears that the sheets themselves transform into vascular spaces and do the healing to the surface in a remodelled fashion. Further, it was observed that there was no blood clot, exudate, or acute inflammatory reaction.
