Figure 3 Calibration plots for glucose in the presence (○) and in the absence (●) of 0.1 mM acetaminophen. The calibrations were recorded with graphite electrodes modified with bienzyme layer of (A) native HRP and glucose oxidase or (B) recombinant HRP and glucose oxidase. The measurements were done in a flow-injection mode, with a flow carrier of 20 mM phosphate buffer, pH 7 containing 0.1M KCl. Applied potential was -50 mV vs. Ag/AgCl/0.1 M KCl.

doi:10.1201/9780203908907-108

Chapter

Figure 3 Calibration plots for glucose in the presence (○) and in the absence (●) of 0.1 mM acetaminophen. The calibrations were recorded with graphite electrodes modified with bienzyme layer of (A) native HRP and glucose oxidase or (B) recombinant HRP and glucose oxidase. The measurements were done in a flow-injection mode, with a flow carrier of 20 mM phosphate buffer, pH 7 containing 0.1M KCl. Applied potential was -50 mV vs. Ag/AgCl/0.1 M KCl.

Chapter

ABSTRACT

The majority of the examples mentioned here were based on DET of peroxidases and especially DET of HRP, the most commonly used peroxidase. However, because native HRP exhibits limited direct electronic coupling with electrodes, the principle of MET has been most frequently exploited for design of HRP-modified electrodes. A good example is the phenothiazine-based HRP-modified electrode with detection limit for H2O2 below 1 nM [87].