![was given at 1, 5, 10, 30, or 60 s after the first pulse. Inset: Rate of recovery measured as the height of the second pulse relative to the first pulse for the control response (○; n=5) and the response in the presence of 2 µM sulpiride (●; n= 4). Solid lines in the inset are double exponentials. For data with sulpiride present, the relative percentage was 100×[1/2 exp (−1.1t)+1/2 exp (−0.15t)]. For control data the relative percentage was 100×[1/2 exp (−1.1t)+1/2 exp (−0.4t)]. (Reproduced from Journal of Neurochemistry with permission [6].)](https://images.tandf.co.uk/common/jackets/crclarge/978082470/9780824707316.jpg "was given at 1, 5, 10, 30, or 60 s after the first pulse. Inset: Rate of recovery measured as the height of the second pulse relative to the first pulse for the control response (○; n=5) and the response in the presence of 2 µM sulpiride (●; n= 4). Solid lines in the inset are double exponentials. For data with sulpiride present, the relative percentage was 100×[1/2 exp (−1.1t)+1/2 exp (−0.15t)]. For control data the relative percentage was 100×[1/2 exp (−1.1t)+1/2 exp (−0.4t)]. (Reproduced from Journal of Neurochemistry with permission [6].)")
ABSTRACT
Uptake inhibitors, such as cocaine and bupropion, have their action at the DAT and were used to alter the rate of uptake in slices. Their actions as competitive uptake inhibitors were confirmed by their alteration of apparent Km values for the rate of uptake of DA by the DAT, and lack of effect on [DA]p, and Vmax. The Ki for each drug was calculated based on the apparent Km, and the values correspond well to those measured in synaptosomes [47]. In this way, these drugs were investigated and their previously reported action on uptake was confirmed with FSCV in slices.
