![Figure 9 Effects of amphetamine (10 µM) on release and uptake of DA. Measurements were made 30 min after drug application. DA release was evoked in the mouse caudateputamen by a single electrical impulse. Measured concentrations were recorded at 100 ms intervals. Concentrations were measured before drug application (●) and 30 min after 10 µM amphetamine (▲). Solid lines represent simulations obtained with the best-fit parameters: before drug, [DA] =9.0 µM, V=3.5 µM/s,](https://images.tandf.co.uk/common/jackets/crclarge/978082470/9780824707316.jpg "Figure 9 Effects of amphetamine (10 µM) on release and uptake of DA. Measurements were made 30 min after drug application. DA release was evoked in the mouse caudateputamen by a single electrical impulse. Measured concentrations were recorded at 100 ms intervals. Concentrations were measured before drug application (●) and 30 min after 10 µM amphetamine (▲). Solid lines represent simulations obtained with the best-fit parameters: before drug, [DA] =9.0 µM, V=3.5 µM/s,")
ABSTRACT
The detection of electrically evoked DA overflow by FSCV in brain slices provides an excellent paradigm in which to study the mechanisms and kinetics of DA neurotransmission. The microelectrode with FSCV provides the size, selectivity, time response, and sensitivity and represents the most ideal sensor for these measurements available at this time. This tool has enabled dynamic measurements of complex phenomena that regulate both release and uptake of DA directly at its site of action.
