in 105 mM K balanced salt solution), a slow change in the background amperometric response is observed. (B) Amperogram of vesicular exocytosis induced by bathing a cell with stimulating solution (described above), with the electrode placed on top of the cell. Narrow current transients are clearly observed at times corresponding to the first two chemical stimulations. Only background current is observed following a third cell stimulation. It appears that the vesicles responsible for the release of catecholamines are depleted with multiple stimulations on this time scale. (C) A one-second portion of data from the first stimulation in B is displayed here with an expanded time scale. All data were obtained using a 5 µm carbon fiber electrode held at 0.65 V vs. SSCE. (Reproduced from Anal. Chem. with permission [15].)

doi:10.1201/9780203908907-118

Chapter

in 105 mM K balanced salt solution), a slow change in the background amperometric response is observed. (B) Amperogram of vesicular exocytosis induced by bathing a cell with stimulating solution (described above), with the electrode placed on top of the cell. Narrow current transients are clearly observed at times corresponding to the first two chemical stimulations. Only background current is observed following a third cell stimulation. It appears that the vesicles responsible for the release of catecholamines are depleted with multiple stimulations on this time scale. (C) A one-second portion of data from the first stimulation in B is displayed here with an expanded time scale. All data were obtained using a 5 µm carbon fiber electrode held at 0.65 V vs. SSCE. (Reproduced from Anal. Chem. with permission [15].)

Chapter

in 105 mM K balanced salt solution), a slow change in the background amperometric response is observed. (B) Amperogram of vesicular exocytosis induced by bathing a cell with stimulating solution (described above), with the electrode placed on top of the cell. Narrow current transients are clearly observed at times corresponding to the first two chemical stimulations. Only background current is observed following a third cell stimulation. It appears that the vesicles responsible for the release of catecholamines are depleted with multiple stimulations on this time scale. (C) A one-second portion of data from the first stimulation in B is displayed here with an expanded time scale. All data were obtained using a 5 µm carbon fiber electrode held at 0.65 V vs. SSCE. (Reproduced from Anal. Chem. with permission [15].)

ABSTRACT

in 105 mM K+ balanced salt solution), a slow change in the background amperometric response is observed. (B) Amperogram of vesicular exocytosis induced by bathing a cell with stimulating solution (described above), with the electrode placed on top of the cell. Narrow current transients are clearly observed at times corresponding to the first two chemical stimulations. Only background current is observed following a third cell stimulation. It appears that the vesicles responsible for the release of catecholamines are depleted with multiple stimulations on this time scale. (C) A one-second portion of data from the first stimulation in B is displayed here with an expanded time scale. All data were obtained using a 5 µm carbon fiber electrode held at 0.65 V vs. SSCE. (Reproduced from Anal. Chem. with permission [15].)