(A) Distribution generated from control PC12 cells (n=29 cells, 77 total events). (B) Distribution generated from L-DOPA-exposed (100 µM for 1 hr) PC12 cells (n=11 cells, 145 total events). For each data set, the peak current of the anodic wave of all events is matched to its respective calibration plot to determine the detected concentration. All data are collected into bins having increments of 0.25 µM and plotted as the percent of the total number of release events detected. (Reproduced from Anal. Chem. with permission [13].)

doi:10.1201/9780203908907-127

Chapter

(A) Distribution generated from control PC12 cells (n=29 cells, 77 total events). (B) Distribution generated from L-DOPA-exposed (100 µM for 1 hr) PC12 cells (n=11 cells, 145 total events). For each data set, the peak current of the anodic wave of all events is matched to its respective calibration plot to determine the detected concentration. All data are collected into bins having increments of 0.25 µM and plotted as the percent of the total number of release events detected. (Reproduced from Anal. Chem. with permission [13].)

Chapter

(A) Distribution generated from control PC12 cells (n=29 cells, 77 total events). (B) Distribution generated from L-DOPA-exposed (100 µM for 1 hr) PC12 cells (n=11 cells, 145 total events). For each data set, the peak current of the anodic wave of all events is matched to its respective calibration plot to determine the detected concentration. All data are collected into bins having increments of 0.25 µM and plotted as the percent of the total number of release events detected. (Reproduced from Anal. Chem. with permission [13].)

ABSTRACT

Figure 10 is a pictorial representation of the space between the cell and electrode surface (the drawing is not to scale) [31]. Following complete fusion of a spherical intracellular vesicle to the plasma membrane, the surface area of the vesicle creates a disk-shaped pore with a radius of 2rv. The contents of the vesicle diffuse from this disk and spread at an angle, θ, until they reach the surface of the carbon fiber electrode positioned at a distance, h, away from the cell surface. The electrochemical reaction occurs at an area defined by re, which is representative of the size of each detected vesicle. This being the case, the quantity re is related to the vesicle radius and the cell/electrode distance (h) by Eq. (3) [31].