ABSTRACT

of 9 of the 16 cells. The second stimulation and release indicates that the varicosity is still responsive after initial stimulation. (B) Representative data from one of the seven cells that exhibited a delay between the time of stimulation with 105 mM potassium chloride and secretion of DA. The mean vesicle content in both immediate and delayed release is 178 zmol. The 5 µm carbon fiber electrode is held at a potential of 0.65 V vs. SSCE for all measurements. The inset is an example of a typical current transient observed at a varicosity displayed on an expanded time scale. The release time (3 ms rise-time of the current spike) is similar to that observed at an undifferentiated cell (Reproduced from Brain Res. with permission [3].)