ABSTRACT
The ability to combine small volumes with excellent sensitivity has made amperometric detection with RDE ideal for bead-based immunoassays. Direct comparison of the results obtained in the above Bugbead assay with those obtained with FIAEC detection showed that the detection limit of RDE was 2 orders of magnitude better than for FIAEC [53]. The other important feature of RDE is the extremely short detection time that could be reached by combining enzyme turnover with electrochemical detection. The RDE detection time of 2 min in the above Bugbead assay is over 10 times faster than the typical detection time for FIAEC that requires a 20 min enzyme substrate incubation. It is also clear from Figure 15 that the detection time could easily be further reduced. Indeed, this has been accomplished in other assays where the detection time was reduced 30-fold over that of FIAEC detection [50].
