Figure 4 Typical potential step sequence in (A) pulsed amperometric detection (PAD) and (B) integrated pulsed amperometric detection (IPAD) of peptides.

doi:10.1201/9780203908907-165

Chapter

Figure 4 Typical potential step sequence in (A) pulsed amperometric detection (PAD) and (B) integrated pulsed amperometric detection (IPAD) of peptides.

ABSTRACT

Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Tyr, and Val [122]. PED for peptides has only been applied for detection in HPLC [123-128, 130]. Leu-Tyr [127], Met-Leu [127], Leu-Trp [127], tryptic digest of β-Casein A2 [127] glutathione [126, 128, 129], and δ-L-α-aminoadipyl-L-cysteinyl-D-valine [130] have all been determined using PED with detection limits (without on-column preconcentration) typically in the 40 nM1.0 µM range [126, 128, 130].