Figure 3 Hydrodynamic voltammogram for the oxidation of 0.58 µM insulin with a mvRuOx-coated glassy carbon electrode. (From J.A.Cox and T.J.Gray, Anal. Chem. 61, 2462, 1989, with permission of the publisher). The conditions are those in Figure 2 except that the currents are corrected for the component from the oxidation of water.

doi:10.1201/9780203908907-181

Chapter

Figure 3 Hydrodynamic voltammogram for the oxidation of 0.58 µM insulin with a mvRuOx-coated glassy carbon electrode. (From J.A.Cox and T.J.Gray, Anal. Chem. 61, 2462, 1989, with permission of the publisher). The conditions are those in Figure 2 except that the currents are corrected for the component from the oxidation of water.

Chapter

ABSTRACT

The electrocatalytic oxidation of insulin at mvRuOx-coated electrodes was employed in a study by Kennedy et al. [11] to elucidate the mechanism of release of this protein from pancreatic cells. The films were coated on electrodes with dimensions in the 10 µm diameter range. Amperometric measurement of insulin released from single cells by glucose stimulation was successful. The study was limited by the instability of the mvRuOx film at pHs above about 5. Apparently, the oxidized form of mvRuOx slowly dissolves in aqueous solution except at low pH values [11, 12].