ABSTRACT
The electrical connection of reaction centers of redox enzymes to electrodes, through their electrical “wiring” with cross-linked redox polymers, provides enzyme electrodes having no leachable components. It forms the basis for electrodes that can be used in vivo and in flow systems and that can be miniaturized to micrometer dimensions. The three parts of this chapter discuss a subcutaneous glucose anode, an immunosensing cathode, and a DNA-sensing microelectrode. The glucose electrooxidizing anode is an example of an electrode designed for use in vivo; the rotating immunosensing electrode exemplifies a “wired” enzyme electrode operating in a fast-flowing solution, and the DNA-sensor is an example of a “wired” enzyme microelectrode.
