°C, and 57°C after adding the SBP-labeled fully complementary or partially mismatched targets. (A) perfectly matched target; (B) target with a single mismatched base; (C) target with four mismatched bases. The dashed lines represent the best fit of the data to Equation 8. Figure 11 Current-time plot of the catalytic current of a microelectrode coated with the probe-bearing redox polymer. An aliquot of the SBP-labeled target with four mismatched bases was introduced at A, then an aliquot of the SBP-labeled perfectly matching target at B.

doi:10.1201/9780203908907-197

Chapter

°C, and 57°C after adding the SBP-labeled fully complementary or partially mismatched targets. (A) perfectly matched target; (B) target with a single mismatched base; (C) target with four mismatched bases. The dashed lines represent the best fit of the data to Equation 8. Figure 11 Current-time plot of the catalytic current of a microelectrode coated with the probe-bearing redox polymer. An aliquot of the SBP-labeled target with four mismatched bases was introduced at A, then an aliquot of the SBP-labeled perfectly matching target at B.

Chapter

°C, and 57°C after adding the SBP-labeled fully complementary or partially mismatched targets. (A) perfectly matched target; (B) target with a single mismatched base; (C) target with four mismatched bases. The dashed lines represent the best fit of the data to Equation 8. Figure 11 Current-time plot of the catalytic current of a microelectrode coated with the probe-bearing redox polymer. An aliquot of the SBP-labeled target with four mismatched bases was introduced at A, then an aliquot of the SBP-labeled perfectly matching target at B.

ABSTRACT

In summary, a single-base mismatch in an 18-base oligonucleotide can be amperometrically sensed with, and amplified by, a redox polymer-coated microelectrode. The detected current is generated by about 34,000 active and wired copies of the thermostable SBP-labeled hybrid. In the future, a complete gene might be hybridized to the 18-base peptide or deoxyribose oligonucleotide [33, 34] bound to the redox polymer, then the presence of the hybridized and thereby redox polymer bound gene would be queried with an SBP-labeled 18-base sequence hybridized to a different region of the gene.