Figure 5 Series of DM-modified duplexes constructed to evaluate long-range electron transfer through DNA films. (Adapted from Ref. 24.) Figure 6 The presence of a CA mismatch between electrode and intercalator prohibits the electrochemical reduction of crosslinked DM (Ref. 24.)

doi:10.1201/9780203908907-9

Chapter

Figure 5 Series of DM-modified duplexes constructed to evaluate long-range electron transfer through DNA films. (Adapted from Ref. 24.) Figure 6 The presence of a CA mismatch between electrode and intercalator prohibits the electrochemical reduction of crosslinked DM (Ref. 24.)

Chapter

ABSTRACT

In order to exploit the efficiency and sensitivity of DNA-mediated redox reactions in biosensing applications, further improvements in the methodology were required to demonstrate the feasibility of this approach. A practical adaptation of the system would have three features: (1) the ability to use a non-cross-linked redox probe, (2) the ability to detect point mutations within oligonucleotides of varying sequence composition, and (3) the ability to achieve in situ hybridization at the electrode surface. The success obtained in each of these areas [25] indicates that this approach may hold promising applications.