buffer containing no protein on a bare PG electrode; (b) 25 µM Mb purified by ultrafiltration in buffer on bare PG; (c) 20 µm Mb-DDAB film on PG electrode in buffer, no Mb in solution. (Adapted from Ref. 17 with permission. Copyright 1995 American Chemical Society.)

doi:10.1201/9780203908907-91

Chapter

buffer containing no protein on a bare PG electrode; (b) 25 µM Mb purified by ultrafiltration in buffer on bare PG; (c) 20 µm Mb-DDAB film on PG electrode in buffer, no Mb in solution. (Adapted from Ref. 17 with permission. Copyright 1995 American Chemical Society.)

Chapter

ABSTRACT

DDAB films are in a lamellar liquid crystal phase at 25°C. The fluidity of this phase facilitates movement of the protein during voltammetry. In thick films, normal pulse voltammetry (NPV) limiting currents, a direct measure of Dct1/2, [11, 17] gave small NPV limiting currents for Mb-DDAB films at temperatures below the gel-to-liquid crystal phase transition temperature (Tc), where they are