Figure 8 Influence of pH on formal potential for films of Mb-DDAB from CV (○) and SWV (+), Mb-DMPC from CV (□) and SWV (●), and Mb-DLPC from CV (+) and SWV (∆). CV values are averages for scan rates from 0.1 to 2Vs , SWV values are

doi:10.1201/9780203908907-96

Chapter

Figure 8 Influence of pH on formal potential for films of Mb-DDAB from CV (○) and SWV (+), Mb-DMPC from CV (□) and SWV (●), and Mb-DLPC from CV (+) and SWV (∆). CV values are averages for scan rates from 0.1 to 2Vs , SWV values are

Chapter

ABSTRACT

Reflectance-absorbance FT-IR (RAIR) and visible spectroscopy showed that conformation of Mb in the films was controlled by the pH of the external solution. Changes in amide I bands (1700-1600 cm−1) are caused by polypeptide C=O stretching

and reflect conformational changes in secondary structure [36]. Second derivative RAIR spectra provide resolution of the amide I band into overlapped bands for different carbonyl environments, allowing semiquantitative identification of structural features such as α-helix, β-sheet, and disordered regions.