ABSTRACT
Glucose oxidase (GOX) is produced predominantly by fungi as part of an enzyme complex also containing catalase. The best-studied microorganisms are species of Aspergillus (1-3) and Penicillium (4, 5). In Aspergillus the enzymatic complex is located in cell compartments called peroxisomes (6), which results in the need to open the fungal cell walls before the enzyme can be released and purified. The enzyme complex of Penicillium is secreted into the extracellular environment (7). Almost all GOX preparations available on the market are produced by Aspergillus niger. These preparations are either highly or somewhat or completely exempt of catalase (see Sec. II.A.1). This chapter will focus mainly on GOX of Aspergillus niger.
