ABSTRACT

Changes in any of the molecular parameters described in Table 1 can be used as a readout to characterize molecular interactions. In most cases, the same optical and electronic configurations are utilized, merely employing a different algorithm for analysis. This means that all fluorescent parameters noted above can be monitored using the same

detection unit. Some of the fluorescence parameters described in Table 1 can even be monitored simultaneously in a single measurement (multiplexing), resulting in exceptional data quality regarding reproducibility and statistics. A comparison of Evotec’s detection technology with other commonly used fluorescence methodologies is shown in Table 2.