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Figure 6 Principle of time-resolved detection of HTRF. The time delay of 50 µsec allows short-lived background fluorescence and the signal from free XL665 to dissipate. Figure 7 Optical configuration of the Discovery HTRF Microplate Analyzer. A nitrogen laser provides the excitation energy of 337 nm. Simultaneous dual wavelength detection provides measurements at 620 nm and 665 nm after a time delay.
DOI link for Figure 6 Principle of time-resolved detection of HTRF. The time delay of 50 µsec allows short-lived background fluorescence and the signal from free XL665 to dissipate. Figure 7 Optical configuration of the Discovery HTRF Microplate Analyzer. A nitrogen laser provides the excitation energy of 337 nm. Simultaneous dual wavelength detection provides measurements at 620 nm and 665 nm after a time delay.
Figure 6 Principle of time-resolved detection of HTRF. The time delay of 50 µsec allows short-lived background fluorescence and the signal from free XL665 to dissipate. Figure 7 Optical configuration of the Discovery HTRF Microplate Analyzer. A nitrogen laser provides the excitation energy of 337 nm. Simultaneous dual wavelength detection provides measurements at 620 nm and 665 nm after a time delay.
ABSTRACT
Figure 6 Principle of time-resolved detection of HTRF. The time delay of 50 µsec allows short-lived background fluorescence and the signal from free XL665 to dissipate.
