ABSTRACT
The following sections are included to provide insight into how one might best develop and optimize an HTRF assay. HTRF assays are normally performed with only a few steps, mainly additions, so there are a smaller number of considerations when designing an HTRF assay than there may be with a more complicated method. The main considerations for optimizing an HTRF assay are buffer selection, whether label (Eu)K or XL665 should be used for each assay component, and what controls should be run.
