ABSTRACT
Protein microarrays have attracted considerable attention as an alternative and complement to conventional proteomics. With the related technologies rapidly advancing, they promise to provide a more comprehensive picture of an organism’s proteome. Moreover, they will significantly acclerate proteomic discovery because of their distinct high-throughput capability. Protein microarrays will permit the screening of very high numbers of protein-protein or compound-protein interactions simultaneously with a high dynamic range and low detection limits, thus enabling one to create protein abundance profiles from complex biological samples, as extreme as an entire organism, with high sensitivity, giving rise to good proteomic coverage, and in short time. However, in order to achieve these desirable features, functionalized surfaces which fulfill several prerequisites are needed.
