ABSTRACT
For detection and identification of chromatogram zones, in situ techniques are generally employed. As in an analytical disk (1), the information stored in the chromatogram can be used for various detection and identification methods, even successively, because the processes of chromatographic development and detection or identification are independent in both time and space. Detection in HPTLC takes place in the absence of the mobile phase and therefore offers much greater choices than any other chromatographic technique. This means that
1. Multiple subsequent detection of the same chromatogram is possible. In addition to recording, e.g., an absorbance or fluorescence scan using visible or ultraviolet (UV) light, a Fourier transform infrared (FTIR) or Raman spectrum can be recorded, and these methods can be followed by a suitable microchemical reaction or mass spectrometry (MS) to provide additional information.
