ABSTRACT

Conventional methods of quantification of fractions resolved by thin-layer chromatography (TLC) using techniques such as in situ spectrophotometry or photodensitometry are of limited utility for substances that contain weak or no chromophoric groups (1). Such fractions can be conveniently detected and quantified by sensitive vapor-phase detectors that are commonly used in gas chromatography (2, 3). Several systems for quantitative TLC using vapor-phase detectors have become known in recent years.