ABSTRACT
Whereas demand for enzymes is largest from the industrial markets, new growth areas are blossoming in many other areas in biotechnology. Novel use for countless new enzymes is a major source of growth in the recent years. Industrial processes including drug design in the pharmaceutical industry, chiral synthesis, therapeutic applications, food processing, detergents, animal feeds, and fuel alcohol markets are the areas that are bound to demand increased attention and investment in biotechnology. Such a demand has spurred new approaches to large-scale production of stable enzymes. In addition to all of this is the prospect of countless new proteins/enzymes that will be appearing in the public domain in the new era of proteomics. Techniques have been established for largescale production of enzymes after changing their structures by protein engineering techniques such as SDM, gene shuffling, or directed evolution of proteins (1).
