Figure 14 Chromatogram of a sample containing among others free sterols, sterol esters, free fatty acids, and fatty acid glycerol esters. The sample is fortified with certain component groups to show more detail. (Reprinted with permission from AJH Louter, CG Bauer-Plank, GSMJE Duchateau. Lipid Technol July 2002, 87–89.)

doi:10.1201/9780203913413-23

Chapter

Figure 14 Chromatogram of a sample containing among others free sterols, sterol esters, free fatty acids, and fatty acid glycerol esters. The sample is fortified with certain component groups to show more detail. (Reprinted with permission from AJH Louter, CG Bauer-Plank, GSMJE Duchateau. Lipid Technol July 2002, 87–89.)

Chapter

ABSTRACT

For samples containing acylglycerides with a wider fatty acid distribution, severe overlap between the compound classes in the chromatogram occurs. In such samples, which in practice unfortunately means most real samples, a class separation is needed before the sterols or sterol esters can be quantified. This can be done using the HPLC-GC approach described above, where PSs and PSEs are separated from the main fat components (TAGs, MAGs) by means of NPLC.