Figure 9 HPLC profiles of total lipid extracts of brush-border and erythrocyte membranes following incubation with 7-dehydrosterols in a mixed micellar medium. A, brush border extract; B, erythrocyte extract; C, extract of medium prior to incubation. Peak identification: 1, 7-dehydrocholesterol; 2, 7-dehydrocampesterol; 3, 7-dehydrositosterol; 4, 7-dehydrocholesterol acetate (internal standard). Brush borders and erythrocytes were incubated for 30min at 37°C in a medium containing 6.6mM sodium taurocholate, 0.6mM egg lecithin, and 30µM each of the 7-dehydrosterols. HPLC conditions: Hewlett-Packard model 5880 liquid chromatograph equipped with a variable-wavelength UV detector and a

doi:10.1201/9780203913413-40

Chapter

Figure 9 HPLC profiles of total lipid extracts of brush-border and erythrocyte membranes following incubation with 7-dehydrosterols in a mixed micellar medium. A, brush border extract; B, erythrocyte extract; C, extract of medium prior to incubation. Peak identification: 1, 7-dehydrocholesterol; 2, 7-dehydrocampesterol; 3, 7-dehydrositosterol; 4, 7-dehydrocholesterol acetate (internal standard). Brush borders and erythrocytes were incubated for 30min at 37°C in a medium containing 6.6mM sodium taurocholate, 0.6mM egg lecithin, and 30µM each of the 7-dehydrosterols. HPLC conditions: Hewlett-Packard model 5880 liquid chromatograph equipped with a variable-wavelength UV detector and a

Chapter

Figure 9 HPLC profiles of total lipid extracts of brush-border and erythrocyte membranes following incubation with 7-dehydrosterols in a mixed micellar medium. A, brush border extract; B, erythrocyte extract; C, extract of medium prior to incubation. Peak identification: 1, 7-dehydrocholesterol; 2, 7-dehydrocampesterol; 3, 7-dehydrositosterol; 4, 7-dehydrocholesterol acetate (internal standard). Brush borders and erythrocytes were incubated for 30min at 37°C in a medium containing 6.6mM sodium taurocholate, 0.6mM egg lecithin, and 30µM each of the 7-dehydrosterols. HPLC conditions: Hewlett-Packard model 5880 liquid chromatograph equipped with a variable-wavelength UV detector and a

ABSTRACT

Figure 9 HPLC profiles of total lipid extracts of brush-border and erythrocyte membranes following incubation with 7-dehydrosterols in a mixed micellar medium. A, brush border extract; B, erythrocyte extract; C, extract of medium prior to incubation. Peak identification: 1, 7dehydrocholesterol; 2, 7dehydrocampesterol; 3, 7dehydrositosterol; 4, 7dehydrocholesterol acetate (internal standard). Brush borders and erythrocytes were incubated for 30min at 37°C in a medium containing 6.6mM sodium taurocholate, 0.6mM egg lecithin, and 30µM each of the 7dehydrosterols. HPLC conditions: Hewlett-Packard model 5880 liquid chromatograph equipped with a variable-wavelength UV detector and a