reversed-phase Supelcosil LC-18 column (5 µm particle size, 25×0.4cm i.d., Supelco). The sterols were eluted with isocratic methanol-acetonitrile (1:1, v/v) solution at a flow rate of 1ml/min and an oven temperature of 30°C. The sterol peaks were monitored at 265nm. Sample: total lipid extracts in methanol-acetonitrile. (From Ref. 64.) Table 4 A Comparison of the Uptake of ∆ -and

doi:10.1201/9780203913413-41

Chapter

reversed-phase Supelcosil LC-18 column (5 µm particle size, 25×0.4cm i.d., Supelco). The sterols were eluted with isocratic methanol-acetonitrile (1:1, v/v) solution at a flow rate of 1ml/min and an oven temperature of 30°C. The sterol peaks were monitored at 265nm. Sample: total lipid extracts in methanol-acetonitrile. (From Ref. 64.) Table 4 A Comparison of the Uptake of ∆ -and

Chapter

ABSTRACT

HPLC provides an alternative method for the resolution of intact steryl esters. While separations of the esters of a single sterol moiety offer marginal advantages over analyses of the released fatty acids, the resolution of intact esters is indispensable for the determination of the fatty acid esters of mixed sterols.