ABSTRACT
Liquid chromatography involves three basic components: a solid stationary phase, a liquid mobile phase, and a column within which both phases are contained. Liquid chromatography, in its simplest form, relies on gravity to draw liquid eluents through an upright column packed with a commercially available stationary phase. High-Performance Liquid Chromatography (HPLC) is an automated technique which relies on specialized equipment, consisting of an eluent delivery module, a sample application module, a column module, and an analyte detector module. HPLC columns are generally pre-packed with stationary phase and may be connected directly to the system. The analyte detector module is required for the continuous real-time monitoring of the mobile phase as it elutes from the column. Reversed phase chromatography separates proteins and peptides on the basis of their hydrophobicity. Affinity Chromatography is a highly specific method for the separation of proteins/peptides on the basis of particular structural features.
