ABSTRACT
Numerous types of fiberoptic chemical sensors are likely to be useful as transducers in fiberoptic biosensors (FOB). The field of FOB can be divided into two main classes, the intrinsic or non-mediated FOB and the chemically mediated FOB. Single-fiber-based FOB is imperative if measurements are to be performed in small sample volumes, as in many in vivo applications. FOB has been tested with nonreal samples, such as stock solutions in distilled water and/or defined buffers in well-thermostated sensor cells. Sensors are classified according to the type of transducer to point out that choice of an appropriate transducer is of great importance to the performance characteristics of the FOB. Also, the same transducer was used, namely a fiberoptic oxygen sensor based on the quenching of the fluorescence intensity of decacyclene dissolved in silicone rubber. A general drawback using pH optodes as transducers for FOB is the influence of both pH and buffer capacity of the analyte solution to the measured signal.
