ABSTRACT
This chapter discusses column overloading conditions and the role of the column efficiency, packing materials, technical problems encountered with large high-performance liquid chromatography (HPLC) columns, and their solutions. A form of PLC, high-performance PLC (HPPLC), has been gaining popularity, particularly in the pharmaceutical industry. HPPLC, because of its higher column efficiencies and faster solvent velocities, permits more difficult separations to be conducted more quickly. The equipment is of utmost importance for successful application of HPPLC. It is worth noting that the contribution of the packing material is usually quite small, even in the case of reversed-phase chromatography. Modern preparative liquid chromatography appears to be a very powerful purification technique, definitely of value to industry, provided that it is optimized properly. If the column contribution to bandbroadening decreases, thereby increasing column efficiency, the load contribution can be increased.
