ABSTRACT
D. H. Campbell et al. sorbed and diazo-coupled protein antigens (Ags) to various supports. Antibodies (Abs), in antiserum added to a column of the cellulose-Ag conjugate, bound to the Ag. Most mammalian cell lines are assumed to contain viral material and oncogenes in the genome. Murine monoclonal Abs are assumed to contain any of a number of murine viruses unless testing precludes their presence. In addition to immunodeficiency virus, human-source factor VIII might also contain hepatitis A virus, hepatitis virus B and hepatitis C virus, together with a variety of other, less virulent species. Viruses can be excluded from solutions of proteins by ultrafiltration. This approach can work, but high losses of F-VIII are possible. Viruses in solution are killed by heat. The diazo coupling utilized in the 1950s was in small-scale commercial use for research in 1965, but by far the most popular coupling technique has been the cyanogen bromide technique developed by A.
