ABSTRACT
For transplantation, several limitations, such as donor site morbidity, infection, inflammation, and immune rejection, remain a challenge in clinical medicine. Three-dimensional (3D) printing and bioprinting techniques have been widely utilized in tissue engineering and could be a way to overcome such clinical limitations. This chapter mainly covers 3D printing techniques, bioink, and applications for tissue regeneration. Biofabrication strategies, such as inkjet-based biofabrication, laser-based biofabrication, and extrusion-based 3D printing, have been introduced. Furthermore, the development of various biomaterial inks with functions such as drug or cell delivery has attracted attention. In particular, decellularized extracellular matrix-based biomaterials are in the global spotlight as a promising source of bioink for tissue regeneration because they can provide inherent tissue functions by recapitulating the composition and components of the native tissue microenvironment with high cellular compatibility.
