ABSTRACT
Aqueous gel permeation high-performance liquid chromatography (HPLC) was introduced and has been a widely accepted technique in protein separation. In many separations, TSKgel SW was used successfully, however, its application in the field of nucleic acid has been very limited so far. G2000SW and G3000SW can easily separate components differing by more than 10% in molecular weight in less than 1 h. Consequently, it may be possible to adopt gel permeation HPLC on TSKgel SW as an alternative to gel electrophoresis, which has been the most popular technique to separate nucleic acids. The main source of variation of elution volume with eluent ionic strength is probably the repulsive ionic interaction between samples and supports, because both nucleic acids and TSKgel SW are negatively charged. Gel permeation HPLC on TSKgel SW was found to be useful for the separation of RNAs and double-stranded DNA fragments.
