ABSTRACT
Determination of the molecular weight of proteins has been one of main subjects in the characterization of proteins. To discover protein molecular weights, researchers had relied heavily upon physicochemical techniques such as ultracentrifugal analysis, light scattering photometry and osmometry. P. Debye first suggested that the molecular weight of a polymer can be determined by the measurement of scattered light. However, P. Putzeys and J. Brosteaux had indicated that the light scattering intensities of several proteins were closely correlated to their molecular weights. M. Fukutomi et al. illustrated that molecular weights of proteins can be determined efficiently by monitoring their elution from a TSK-GEL SW GPC column. T. Takagi further assessed the performance of the technique. For proteins without molecular weight distribution, the height of each peak can be taken as a measure of the output of the corresponding detector.
