ABSTRACT
DNA markers such as restriction fragment length polymorphisms (RFLP) enable the development of large numbers of genetic markers useful for the construction of genetic linkage maps, and for systematic analysis of quantitative trait loci (QTLs). Allopolyploidy in sugarcane has been indicated by RFLP segregation data of progeny derived from a cultivar11 and a wild sugarcane species, necessitating linkage analysis using single dosage restriction fragments. However, methods based on the segregation of diploid populations are not readily applicable for QTL mapping in polyploids, due to the unique genetic characteristics of polyploids. As a fundamental requirement for detecting QTLs, the mapping population must demonstrate segregation at the QTLs, as evidenced by showing significant genetic variation for the quantitative trait. The power of QTL detection may be increased by analyzing phenotypic data collected from multiple years or locations to minimize the environmental variance. Further, selective genotyping of the progeny with extreme phenotypes can improve the efficiency of QTL detection.
