ABSTRACT
In Dermatotoxicology, human percutaneous absorption determines the amount of chemical that goes from external exposure to systemic circulation. This chapter presents the more relevant ways to determine human percutaneous absorption. Percutaneous absorption in vivo is usually determined indirectly by measuring radioactivity in excreta topical application of the labeled compound. The methodology is to surgically isolate a portion of skin so that a singular blood supply is created to collect blood containing the chemical that has been absorbed through skin. Radioactivity in blood and excreta can include both the applied compound and metabolites. There are two components to skin washing in the recovery of chemicals. The first component is the physical rubbing and removal from the skin surface. The second component is the solvent action of soap and water. Glyphosate is a water-soluble compound. There are a variety of factors that affect human percutaneous absorption.
