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Chapter

Light Field Microscopy for In Vivo Ca2+ Imaging

Chapter

Light Field Microscopy for In Vivo Ca2+ Imaging

DOI link for Light Field Microscopy for In Vivo Ca2+ Imaging

Light Field Microscopy for In Vivo Ca2+ Imaging book

Light Field Microscopy for In Vivo Ca2+ Imaging

DOI link for Light Field Microscopy for In Vivo Ca2+ Imaging

Light Field Microscopy for In Vivo Ca2+ Imaging book

ByTobias Nöbauer, Alipasha Vaziri
BookHandbook of Neurophotonics

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Edition 1st Edition
First Published 2020
Imprint CRC Press
Pages 32
eBook ISBN 9780429194702

ABSTRACT

Light Field Microscopy is an emerging technique for neuroimaging with a high potential for dissemination in the neuroscience community. The general idea of recording and manipulating light fields is far-reaching and continues to inspire a wealth of new developments and variations. The concept of fully capturing the direction and position of all light rays propagating in a volume is known as plenoptic imaging, and the quantity containing all the captured information is the plenoptic function. For many applications, including Ca2+ imaging, it is necessary to reconstruct the 3D distribution of light sources in order to find the positions of the individual cells and to demix their signals from each other and any background. Light scattering in larval zebrafish brain is much weaker than, e.g., in the mammalian cortex, but severe enough to lead to noticeable blurring when imaging structures across the brain.

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