ABSTRACT
The method transfer between conventional high-performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC) is done either to accelerate the method or to gain more resolution compared to the HPLC method. The goals for HPLC method transfer to an UHPLC method can focus on either maintaining or improving the HPLC separation efficiency, or accelerating the HPLC method to increase sample throughput. One motivation for the transfer from an HPLC to UHPLC method is to obtain better separation of the analytes. For successful HPLC to UHPLC method transfer, an adequate column packed with smaller particles has to be carefully selected. The column chemistry and selectivity of the stationary phase should be maintained to get the closest prediction of the UHPLC method. The extra-column volume plays an important role for the HPLC to UHPLC method transfer. The method transfer process from HPLC to UHPLC considering all the geometric rules can be easily integrated into calculators.
