ABSTRACT
In achiral chromatography, there are many understood interactions and equilibria that take place between the analytes, the solid phase of the column and the liquid phase. These interactions can be modeled and often fall within well established theories and equations of chromatographic separations. One tool available to aid in chiral analyses is detectors that differentiate between enantiomeric pairs and provide unique, distinguishable signals for each enantiomeric compound. In the absence of a detector that can differentiate between enantiomers, the analyst is left with only a diode array detector or a mass detector to identify chiral pairs. Normal light waves vibrate in many planes; however, plane polarized light is generated when normal light is passed through an optical polarizing filter. This effect results in a light beam emerging that vibrates in a single plane. The plain optical rotation dispersion curve shows the relationship between the magnitude of the optical rotation versus wavelength.
